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Correlation study between standard plate count and flow cytometry for determination of raw milk total bacterial count
Author(s) -
CASSOLI LAERTE DAGHER,
MACHADO PAULO FERNANDO,
DE OLIVEIRA RODRIGUES ANA CAROLINA,
COLDEBELLA ARLEI
Publication year - 2007
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/j.1471-0307.2007.00297.x
Subject(s) - plate count , raw milk , flow cytometry , somatic cell count , count data , zoology , standard deviation , total viable count , bacterial colony , mathematics , veterinary medicine , biology , statistics , food science , bacteria , immunology , medicine , pregnancy , lactation , ice calving , poisson distribution , genetics
Milk samples were collected in the dry season ( n = 155) and in the rainy season ( n = 68) to establish a correlation between electronic flow cytometry and standard plate count for the determination of total bacterial count of raw milk. Results were expressed in individual bacterial count (ibc) and colony forming unit (cfu) for electronic flow cytometry (Bactocount) and standard plate count, respectively. The accuracy of Bactocount, denoted by the residual standard deviation ( s ( y,x )), was 0.309 log cfu/mL. The predictive interval of estimated values was broad and it is suggested that total bacterial count should be expressed in ibc without transformation to cfu.