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Comparison of two new methods for the detection of bacterial proteases
Author(s) -
SUTHERLAND A D
Publication year - 1993
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/j.1471-0307.1993.tb00857.x
Subject(s) - proteases , protease , pseudomonas fluorescens , casein , chemistry , raw milk , chromatography , bioluminescence , biochemistry , fluorescein isothiocyanate , fluorescence , microbiology and biotechnology , food science , biology , enzyme , bacteria , physics , quantum mechanics , genetics
Bioluminescence and fluorimetric fluorescein isothiocyanate‐casein based assays were compared for their ability to detect bacterial proteases in buffer and milk. Both assays gave linear relationships with protease concentration. The fluorimetric assay was overall 1000 times more sensitive than the bioluminescence assay. Only the fluorescent assay was capable of detecting protease activity produced in raw milk inoculated with Pseudomonas fluorescens and stored at 6°C. The protease activity was detectable in raw milk before physical changes occurred and was also detectable after a laboratory ultra high temperature treatment. Use of the fluorimetric assay is a promising means of detecting protease activity in raw materials before manufacture