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Performance of a rapid ELISA for penicillin G in milk
Author(s) -
JACKMAN R,
CHESHAM J,
MITCHELL S J,
DYER S D
Publication year - 1990
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/j.1471-0307.1990.tb02455.x
Subject(s) - penicillin , false positive paradox , antimicrobial , confidence interval , chromatography , detection limit , food science , chemistry , microbiology and biotechnology , medicine , antibiotics , biology , mathematics , statistics
A rapid enzyme‐linked immunosorbent assay (ELISA) for the detection of penicillin G in milk at concentrations of 6 ng/ml (0.01 IU/ml) was used to screen 1651 off‐farm milk samples previously reported as containing no detectable levels of antimicrobial substances. Using a single‐well per test format and a percent inhibition cut‐off for the determination of positive/negative endpoint, 3.1% positives were obtained. Comparison with intra‐assay standards lowered the numbers of positives to 0.42%. Analysis of 170 milk samples positive for antimicrobials confirmed 92.4% as containing penicillin G using the prescribed cut‐off level and 87.6% when compared to standards. The ELISA, now available commercially in kit form, analyses between 1 and 48 samples in 15 minutes. It will detect 0.01 IU/ml to a level of confidence of 99% and 0.005 IU/ml with between 75 and 95% confidence.