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Collaborative trial of the Penzym assay: a rapid method for the detection of β ‐lactam antibiotics in milk
Author(s) -
KNIGHT ANABEL H,
SHAPTON NORAH,
PRENTICE GEORGE A
Publication year - 1987
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/j.1471-0307.1987.tb02831.x
Subject(s) - antibiotics , penicillin , raw milk , chromatography , medicine , chemistry , microbiology and biotechnology , biology , pathology
The Penzym assay is a rapid enzymic method for the detection of β‐lactam antibiotics in milk in 20 minutes. Two collaborative trials of the Penzym assay were conducted between 11 laboratories using raw whole milk samples containing levels of 0.005 iu/ml, 0.015 iu/ml and 0.025 iu/ml penicillin G and were tested at 0.01 iu/ml and 0.02 iu/ml pass/fail levels. Trial 1 indicated a degree of operator dependency with a tendency to give false passes. Trial 2 confirmed the operator dependency, and both false passes and false failures were obtained. These results make the test unacceptable as a rejection test for detecting β‐lactam antibiotics in dairy laboratories. However, given the speed of testing and level of accuracy (92%), the assay may still be valuable as a screening test .