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Isolation and identification of proteolytic psychrotrophic sporeforming bacteria from raw milk supplies at an experimental dairy in India
Author(s) -
SHARMA J K,
MALIK R K,
MATHUR D K
Publication year - 1984
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/j.1471-0307.1984.tb00499.x
Subject(s) - raw milk , protease , bacilli , cereus , food science , pasteurization , psychrotrophic bacteria , biology , bacillus coagulans , bacillus subtilis , isolation (microbiology) , proteolytic enzymes , microbiology and biotechnology , bacillus cereus , bacteria , enzyme , biochemistry , genetics , fermentation
Of 51 samples of raw milk produced at the National Dairy Research Institute, 65% were found to contain proteolytic psychrotrophic sporeforming bacilli. The protease activity of 50 isolates selected ranged from 20 to 480 units/ml. Twenty/four per cent (12) of the total isolates exhibited enzyme activity in the range 51–100 units/ml, while 20% (10) had protease activity of more than 300 units/ml. Of the 50 isolates, 36% were Bacillus cereus, 20% B. polymyxa, 14% each B. laterosporus and B. circulans. 10% B. pumilus. 4% B. subtilis and 2% were identified as B. coagulans.

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