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Recent developments and the use of miniaturized tests for the detection of inhibitory substances in milk
Author(s) -
GILMOUR A.,
HAMILTON W. J.
Publication year - 1981
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/j.1471-0307.1981.tb02598.x
Subject(s) - penicillin , raw milk , screening test , chromatography , raw material , antibiotics , chemistry , food science , medicine , biochemistry , family medicine , organic chemistry
The work described was undertaken to assess the suitability of raising the incubation temperature of the miniaturized Intertest and Oxoid (AIM) test from 37.5°C to 45°C and to investigate the use of Intertest Accuspheres as a material suitable for a miniaturized screening test. Fresh raw bulk antibiotic‐free volumes of milk were used, and to these were added appropriate volumes of a standard penicillin solution to give various concentrations of penicillin. Standard and miniaturized tests were carried out at 37.5°C and 45°C (Accuspheres tests were carried out at 45°C only). The results of tests carried out at 45°C show that, although complete agreement between the standard and miniaturized tests was not obtained, a definite blue colour, interpreted as a fail, always resulted when milk tested contained ≥0.02 IU/ml added penicillin. It is considered that all three miniaturized tests, carried out at 45°C, are suitable for use as screening tests for the detection of inhibitory substances in milk.