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Towards molecular farming in the future: transient protein expression in plants
Author(s) -
Fischer Rainer,
VaqueroMartin Carmen,
Sack Markus,
Drossard Jürgen,
Emans Neil,
Commandeur Ulrich
Publication year - 1999
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1999.tb00900.x
Subject(s) - agroinfiltration , recombinant dna , transient (computer programming) , genetically modified crops , transgene , protein expression , computational biology , biology , microbiology and biotechnology , expression (computer science) , gene expression , biochemistry , computer science , gene , programming language , operating system
Molecular farming in plants can be achieved by stable or transient expression of a recombinant protein. Transient expression of recombinant proteins in plants can rapidly provide large amounts of the proteins for detailed characterization. It is fast, flexible and can be carried out at field scale using viral vectors, but it lacks the increases in production volume that can be achieved easily with stable transgenic crops. This review article focuses on discussing the applications of transient expression using viral vectors, biolistic methods or agroinfiltration.