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Immobilization of cholesterol oxidase on Formvar using organic solvents
Author(s) -
Kumar Hemant,
Kumar Ashok,
Kumari Poonam,
Jyotirmai S.,
Tulsani N. B.
Publication year - 1999
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1999.tb00775.x
Subject(s) - membrane , immobilized enzyme , chemistry , cholesterol oxidase , enzyme , chromatography , organic solvent , enzyme assay , organic chemistry , chemical engineering , biochemistry , engineering
The present study describes a new immobilization support for the preparation of enzyme membrane in the presence of organic solvents. The support was composed of Formvar solubilization in organic solvents with enzyme. More than 90% of the enzyme was immobilized on the membrane. The membrane was prepared by mixing 4% Formvar in organic solvents and 1% cholesterol oxidase was immobilized by entrapment technique. Practically no leaching of the entrapped enzyme was observed. The resultant immobilized enzyme membrane was stored at 4 °C for 10 days without losing its activity. The pH and temperature stabilities were greater than those of the native enzyme. The immobilized enzyme membrane has a long life due to its hydrophobic nature, as compared with other membranes.