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Maximization of cholesterol oxidase production by Rhodococcus equi no. 23 by using response surface methodology
Author(s) -
Lee MingTsung,
Chen WenChang,
Chou ChengChun
Publication year - 1998
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1998.tb00534.x
Subject(s) - cholesterol oxidase , rhodococcus equi , cholesterol , response surface methodology , yeast extract , yeast , chemistry , biochemistry , central composite design , oxidase test , food science , enzyme , chromatography , fermentation , virulence , gene
Medium optimization for the production of cholesterol oxidase (EC 1.1.3.6) by Rhodococcus equi no. 23 was investigated by using response surface methodology and a central composite design. Results revealed that cholesterol and yeast extract had positive effects and the interaction between any two of three factors had no significant effect on cholesterol oxidase production. The optimized medium was the basal medium with the addition of 2.30 g/l cholesterol, 8.18 g/l yeast extract and 4.10 ml/l Tween 80. The peak cholesterol oxidase production (0.242 unit/ml) after 60–72 h cultivation was approx. 4‐fold that in control medium.