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Purification and characterization of lipase from a raw‐milk yeast ( Trichosporon asteroides )
Author(s) -
Dharmsthiti Saovanee,
Ammarad Palanee
Publication year - 1997
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1997.tb00455.x
Subject(s) - lipase , sephadex , yeast , chromatography , chemistry , size exclusion chromatography , docosahexaenoic acid , triacylglycerol lipase , biochemistry , eicosapentaenoic acid , fatty acid , enzyme , polyunsaturated fatty acid
A lipase‐producing yeast strain was isolated from raw milk. It was identified as Trichosporon asteroides strain LP005. The lipase from this yeast was purified 8‐fold to homogeneity for further characterization. The purification process for this lipase included (NH 4 ) 2 SO 4 precipitation at 70% saturation and gel filtration on Sephadex G‐200. The molecular mass of the lipase was 37 kDa as determined by SDS/PAGE. The optimum pH and temperature for activity were pH 5.0 and 60 ° C. The lipase was stable over a wide pH range (3.0Ő10.0) and at temperatures lower than 70 ° C. The chelating agent EDTA did not affect activity of the enzyme, and this suggested that it was not a metalloenzyme. Treatment of tuna oil with T. asteroides LP005 lipase gave approx. 35 and 47′ increases in the concentration of eicosapentaenoic acid and docosahexaenoic acid respectively. Thus, this lipase could potentially be used for the concentration step in the production process of such polyunsatuarated fatty acids.