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Some biochemical properties and the classification of a range of bacterial haloalkane dehalogenases
Author(s) -
Damborsky Jii,
Nyandoroh Munyaradzi G.,
Mec Miroslav N,
Holoubek Ivan,
Bull Alan T.,
Hardman David J.
Publication year - 1997
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1997.tb00442.x
Subject(s) - substrate specificity , stereochemistry , chemistry , enzyme , biochemistry , biology
Multivariate analyses and experimental data have been used to evaluate the relationships between eight bacterial hydrolytic haloalkane dehalogenases. The results indicate that seven of the dehalogenases investigated can confidently be placed into two Classes [ sensu Slater, Bull and Hardman (1995) Biodegradation 6, 181Ő189] according to their substrate profiles. The remaining enzyme, isolated from Rhodococcus erythropolis CP9, appears to represent a third Class of haloalkane dehalogenases.