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Polylysine enhances cationic liposome‐mediated transfection of the hepatoblastoma cell line Hep G2
Author(s) -
KD Mack,
RL Walzem,
LehmannBruinsma K.,
JS Powell,
JB Zeldis
Publication year - 1996
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1996.tb00378.x
Subject(s) - polylysine , cationic polymerization , cationic liposome , transfection , liposome , hep g2 , chemistry , luciferase , cytotoxicity , microbiology and biotechnology , cell culture , phospholipid , biochemistry , hepatoblastoma , lipofectamine , biology , in vitro , recombinant dna , medicine , vector (molecular biology) , polymer chemistry , membrane , gene , genetics
Plasmid DNA condensed by polylysine enhanced cationic‐liposome‐mediated transfection of Hep G2 cells. The luciferase expression plasmid pCMVL was complexed with the polycation poly‐L‐lysine and mixed with liposomes that contained a 1:1 molar ratio of the cationic lipid 1,2‐dioleoyloxy‐3‐trimethyl‐ammoniumpropane, with the neutral phospholipid 1,2‐di‐(cis‐9‐octadecenoyl)‐sn‐glycero‐3‐phosphoethanolamine. Polylysine enhanced cationic‐liposome‐mediated transfection of the hepatoblastoma cell line Hep G2 9‐fold compared with pCMVL complexed alone with liposomes. The ratio of cationic to anionic charge of the polylysine‐pCMVL complexes, and the quantity of cationic liposomes, are important determinants for optimal transfection of Hep G2 cells.