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Correlation of the OSR/ZRCI gene product and the intracellular glutathione levels in Saccharomyces cerevisiae
Author(s) -
Kobayashi S.,
Miyabe S.,
Izawa S.,
Inoue Y.,
Kimura A.
Publication year - 1996
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1996.tb00356.x
Subject(s) - mutant , saccharomyces cerevisiae , glutathione , gene , gene product , biology , biochemistry , gene knockout , microbiology and biotechnology , oxidative stress , intracellular , gene expression , enzyme
The OSR (oxidative‐stress‐resistance) gene was cloned as the gene which enhances resistance against oxidative stress caused by lipid hydroperoxide in Saccharomyces cerevisiae. The nucleotide sequence of the gene was determined and found to be identical with the zinc‐resistance‐conferring (ZRCI) gene, which was cloned as the gene enhancing the resistance against zinc and cadmium. A knockout mutant of the OSR gene showed increased sensitivity to lipid hydroperoxide. The intracellular glutathione content in the knockout mutant decreased approx. 40% compared with that of wild‐type cells, whereas it increased 3‐fold in yeast overexpressing the OSR gene. Therefore the OSR/ZRCI gene product seems to regulate the intracellular glutathione content.