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Heterologous gene expression and protein secretion from Candida glabrata
Author(s) -
Macreadie I.,
Castelli L.,
Mehra R.,
Thorvaldsen J.,
Winge D.
Publication year - 1994
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1994.tb00297.x
Subject(s) - candida glabrata , heterologous expression , biology , secretion , heterologous , saccharomyces cerevisiae , gene , microbiology and biotechnology , escherichia coli , recombinant dna , biochemistry , candida albicans
We have examined heterologous protein secretion from Candida glabrata with the aid of a stable C. glabrata vector and a secretion reporter cassette comprising the Saccharomyces cerevisiae PGK‐gene promoter and a Kluveromyces Iactis secretion signal to drive secretion of Escherichia coli beta‐lactamase. Abundant secretion of beta‐lactamase from C. glabrata indicates that the S. cerevisiae PGK promoter functions in C. glabrata. Furthermore, we show that C. glabrata processes the secreted beta‐lactamase in a manner similar to, but not identical with, S. cerevisiae and K. lactis. C. glabrata may be a suitable new host for the expression of foreign genes.