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Purification of endo‐polygalacturonase by affinity precipitation using alginate
Author(s) -
Gupta MN,
Dong G.,
Mattiasson B.
Publication year - 1993
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1993.tb00275.x
Subject(s) - pectinase , enzyme , aspergillus niger , precipitation , chromatography , chemistry , specific activity , enzyme assay , biochemistry , physics , meteorology
The precipitation of alginate by Ca2+ at pH 3.8 was found to occur concomitantly with the precipitation of endo‐polygalacturonase from Aspergillus niger. Under optimum conditions, 92% of the enzyme activity was precipitated. The enzyme could be recovered from the precipitate by washing with 0.5 M NaCl/0.2 M Ca2+. All the precipitated endo‐polygalacturonase activity could be recovered in this way. The enzyme thus obtained was purified 10‐fold. A comparison of SDS/PAGE gel patterns of the crude preparation and enzyme purified by the affinity precipitation also showed a significant purification of the enzyme.