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mRNA‐ribosome interactions
Author(s) -
Dokudovskaya SS,
Dontsova OA,
Bogdanova SL,
Bogdanov AA,
Brimacombe R.
Publication year - 1993
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1993.tb00261.x
Subject(s) - ribosome , ribosomal rna , messenger rna , start codon , ribosomal protein , transfer rna , shine dalgarno sequence , ribosomal binding site , microbiology and biotechnology , transcription (linguistics) , 30s , chemistry , eukaryotic ribosome , biology , biochemistry , rna , gene , linguistics , philosophy
Synthetic mRNA analogues were constructed with sequences related to the Cro‐protein mRNA from lambda‐phage and prepared by T7 transcription. Each mRNA contained several thiouridine (thio‐U) residues. The regions upstream from the AUG initiator codon of the mRNA were the same in all the messages, whereas in the downstream part the thio‐U residues were placed in selected positions. These positions covered the region from +4 to +16 (A in the initiator AUG codon being defined as +1). After binding to the ribosome in the presence of initiator tRNA the thio‐U residues were activated by u.v. irradiation and the resulting sites of cross‐linking to 16 S rRNA and ribosomal proteins were analysed. Cross‐links to several ribosomal proteins were identified in different types of complex. Changes in the conformation of the small ribosomal subunit in different initiation and elongation complexes are discussed.