Premium
Immobilization of beta‐glucosidase from Penicillium funiculosum on nylon powder
Author(s) -
Aguado J.,
Romero MD,
Rodriguez L.
Publication year - 1993
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1993.tb00232.x
Subject(s) - glutaraldehyde , tetrafluoroborate , chemistry , nuclear chemistry , enzyme , immobilized enzyme , chromatography , organic chemistry , catalysis , ionic liquid
beta‐Glucosidase from Penicillium funiculosum was immobilized on nylon powder previously activated with triethyloxonium tetrafluoroborate, 1,2‐diaminoethane and glutaraldehyde. The activation of the nylon powder and the immobilization processes were studied and optimized for the enzyme and the matrix. A high activity retention (67%) was obtained using the activation and immobilization conditions finally selected.