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Evaluation of collagen cross‐linking techniques for the stabilization of tissue matrices
Author(s) -
Simmons DM,
Kearney JN
Publication year - 1993
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1993.tb00229.x
Subject(s) - azide , glutaraldehyde , chemistry , succinic anhydride , dermis , calcification , cytotoxicity , biochemistry , lysine , organic chemistry , in vitro , anatomy , biology , pathology , medicine , amino acid
The use of glutaraldehyde (GTA) for cross‐linking biological tissue implants has a number of undesirable side effects, including cytotoxicity and induction of calcification. In an attempt to find an improved cross‐linking agent for tissue implants, we have evaluated a number of cross‐linking procedures shown previously to be effective with pure collagen or collagenous substrates, including GTA, succinic anhydride, cyanamide, 1‐ethyl‐3‐(dimethylaminopropyl)carbodi‐imide, dimethyl suberimidate, ascorbate‐copper, glucose‐lysine and acyl azide treatments. Apart from GTA, only acyl azide treatment significantly cross‐linked human dermis, the degree of cross‐linking being better than that seen after treatment of dermis with 1 mM GTA. Acyl azide treatment of thicker vascular tissue (porcine aorta) also resulted in a significantly cross‐linked tissue. Preliminary cytotoxicity studies suggested that acyl azide treatment was not toxic, and, therefore, coupled with its cross‐linking ability, this treatment is worthy of further investigation and may prove to be an alternative to GTA for the treatment of bioprostheses.