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Immortalization of human aortic smooth muscle cells with origin‐minus simian virus 40 DNA
Author(s) -
Murahashi N.,
Sasaguri Y.,
Ohuchida M.,
Kakita N.,
Morimatsu M.
Publication year - 1992
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1992.tb00219.x
Subject(s) - collagenase , cell culture , matrix metalloproteinase , transfection , extracellular matrix , immortalised cell line , fibronectin , gelatinase , gelatinase a , biology , basement membrane , cell , microbiology and biotechnology , cell type , secretion , genetics , enzyme , biochemistry
We established two cell lines of human smooth muscle cells (SMC) by transfection of cells from the aortic intima and aortic media with origin‐minus simian virus 40 (ori‐minus SV40) DNA. Ori‐minus SV40 DNA very efficiently immortalized human smooth muscle cells in culture. Proteins that these cell lines produced included type I, III, IV, and V collagens, fibronectin, and human matrix metalloproteinases (MMP)−1 (tissue collagenase), −2 (“type IV collagenase”), and −3 (stromelysin). The protein production in these cell lines generally mimicked that of normal SMC, but the immortalization stimulated the cell line of medial SMC to produce excessive MMP‐2 and to secrete MMP‐9 (92‐kDa gelatinase). However, since these cell lines did not show a fully malignant phenotype, we concluded that, in addition to the degradation of extracellular matrix macromolecules, including basement membrane components by MMP‐2, −3, and/or −9, some additional factors must be involved for the malignancy of fully transformed cells and that these immortalized human aortic SMC, which share many characteristics with normal SMC, will prove useful to study the role(s) of metalloproteinases in atherosclerosis.