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Cloning and expression of the Erwinia herbicola tyrosine phenol‐lyase gene in Escherichia coli
Author(s) -
Iwamori S.,
Oikawa T.,
Ishiwata K.,
Makiguchi N.
Publication year - 1992
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1992.tb00214.x
Subject(s) - citrobacter freundii , escherichia coli , gene , nucleic acid sequence , cloning (programming) , biology , microbiology and biotechnology , tyrosine , peptide sequence , biochemistry , gene expression , erwinia , amino acid , enterobacteriaceae , computer science , programming language
The tyrosine phenol‐lyase (TPL) gene of Erwinia herbicola was cloned and expressed in Escherichia coli, and the complete nucleotide sequence of the gene determined. The TPL gene comprises 1368 bp, encoding 456 amino acids which have 90% amino acid identity with TPL from Citrobacter freundii. After replacing the 5′‐flanking region of the TPL gene with the E. coli lac promoter, TPL protein could be hyperproduced constitutively in E. coli without induction by L‐tyrosine.