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Positive effect of GAC gene product on the mRNA level of glyoxalase I gene in Saccharomyces cerevisiae
Author(s) -
Inoue Y.,
Yano H.,
Ginya H.,
Tsuchiyama H.,
Murata K.,
Kimura A.
Publication year - 1991
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1991.tb00193.x
Subject(s) - lactoylglutathione lyase , saccharomyces cerevisiae , gene product , yeast , gene , biochemistry , biology , gene expression , microbiology and biotechnology , chemistry , enzyme , methylglyoxal
A DNA fragment carrying a part of the structural gene for yeast (Saccharomyces cerevisiae) glyoxalase I was cloned from a lambda gt11 expression library using anti‐glyoxalase IIgG as a probe. By Northern blotting analysis, the amount of glyoxalase I mRNA was found to increase in yeast cells containing plasmids carrying the GAC gene, which is a positive regulator for yeast glyoxalase I activity. This suggests that the GAC gene product may accelerate the transcription of glyoxalase I gene or may have some positive effects on the accumulation of glyoxalase I mRNA in yeast cells.