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Preparative isolation of polyphosphoinositides and other anionic phospholipids from natural sources using chromatography on adsorbents containing primary amino groups
Author(s) -
Klyashchitsky BA,
Mezhova IV,
Krasnopolsky Yu M,
Shvets VI
Publication year - 1991
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1991.tb00181.x
Subject(s) - chemistry , chromatography , adsorption , cardiolipin , phospholipid , ion chromatography , ion exchange , hydrophilic interaction chromatography , high performance liquid chromatography , membrane , organic chemistry , biochemistry , ion
A new method for the preparative isolation of anionic phospholipids with the use of chromatography on adsorbents containing primary amino groups has been developed. The method combines elements of bioaffinity and ion‐exchange chromatography. Synthesis of adsorbents based on Spheron and silica supports with immobilized neomycin, L‐lysine, or aminoalkyl groups was carried out. Optimal conditions for the separation of phospholipid mixtures on aminosorbents were determined. Separation of rat brain bis‐ and trisphosphoinositides and preparative isolation of bovine heart cardiolipin and baker's yeast phosphatidylinositol are described. The chromatographic behavior of anionic phospholipids on three types of adsorbent was studied. The contribution of biospecific interactions to the adsorption of polyphosphoinositides on aminosorbents is noted.