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Intraperitoneal administration of carrier erythrocytes in dogs: an improved method for delivery of L‐asparaginase
Author(s) -
DeLoach JR,
Andrews K.,
Satterfield W.,
Keeling M.
Publication year - 1990
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1990.tb00105.x
Subject(s) - intraperitoneal injection , asparaginase , enzyme , tonicity , inulin , pharmacology , chemistry , dialysis , medicine , biochemistry , lymphoblastic leukemia , leukemia
Carrier erythrocytes were prepared to encapsulate L‐asparaginase by a hypotonic dialysis process. Dogs received either intravenous or intraperitoneal injections of cells containing both L‐asparaginase and the marker [3H]inulin. The route of administration had no effect on the circulating survival of carrier erythrocytes. For enzyme therapy, intraperitoneal injection of a large volume of cells is technically feasible. Cells circulate with a maximum of 30% of the cells reaching circulation 24 h after injection. Those cells reaching circulation have a 7‐day half‐life for the encapsulated enzyme L‐asparaginase.