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Molecular cloning of the cys genes (cysC, cysD, cysH, cysI, cysJ, and cysG) responsible for cysteine biosynthesis in Escherichia coli K‐12
Author(s) -
Tei H.,
Murata K.,
Kimura A.
Publication year - 1990
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1990.tb00093.x
Subject(s) - escherichia coli , gene , regulon , cloning (programming) , mutant , molecular cloning , biology , genetics , restriction map , microbiology and biotechnology , chromosome , structural gene , plasmid , gene expression , computer science , programming language
The cysCDHIJ gene region at 59 min and the cysG gene at 74 min on the chromosome of Escherichia coli K‐12 were cloned. The ability of these cloned genes to complement mutants was analyzed and their restriction maps were determined. The following results were obtained. First, the cysCDHIJ regulon could be separated into cysCD and cysHIJ regions. Second, the cysG gene was quite different from the cysI and cysJ genes.