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Increasing cytostatic effects of ricin A chain and Staphylococcus aureus enterotoxin A through in vitro hydrophobization with fatty acid residues
Author(s) -
Alakhov V Yu,
Kabanov AV,
Batrakova EV,
Koromyslova IA,
Levashov AV,
Severin ES
Publication year - 1990
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1990.tb00083.x
Subject(s) - ricin , enterotoxin , stearic acid , chemistry , fatty acid , residue (chemistry) , toxin , in vitro , micelle , biochemistry , staphylococcus aureus , organic chemistry , bacteria , biology , escherichia coli , genetics , aqueous solution , gene
In order to impart an ability for receptor‐independent transmembrane transfer to water‐soluble proteins, it has been suggested that they be hydrophobized by lipid groups (fatty acids, etc.). To this end, systems of reversed micelles of surfactants in organic solvents were used as reaction media for protein modification. It was shown that after introduction of a hydrophobic anchor (stearic acid residue) the toxic effect of ricin A‐chain (in the absence of B‐chain) on intact cells became very close to that of the native toxin. As a result of stearic acid acylation, the activity of Staphylococcal enterotoxin A increased by nearly 1.5‐2 orders. The observed phenomena can be explained by receptor‐independent intracellular translocation of the hydrophobized toxins.