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Selective adsorption of apoferritin on immobilized Fe(III): demonstration of Fe(III) binding sites
Author(s) -
Boyer RF,
Generous SM,
Nieuwenhuis TJ,
Ettinger RA
Publication year - 1990
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1990.tb00081.x
Subject(s) - chemistry , adsorption , metal , covalent bond , ionic strength , ionic bonding , metal ions in aqueous solution , ferritin , inorganic chemistry , affinity chromatography , ion chromatography , chromatography , ion , organic chemistry , biochemistry , aqueous solution , enzyme
Immobilized metal ion affinity chromatography has been used to demonstrate and partially characterize Fe(III) binding sites on apoferritin. Binding of Fe(III) to these sites is influenced by pH, but not affected by high ionic strength. These results suggest that both ionic and coordinate covalent interactions are important in the formation of the Fe(III): apoferritin complex. This is, to our knowledge, the first demonstration of direct Fe(III) binding to apoferritin. Other immobilized metal ions, including Zn(II), Ni(II), Cu(II), Cr(III), Co(II), and Tb(III), displayed little or no adsorption of apoferritin. The analytical technique of immobilized metal ion affinity chromatography also shows great promise in the purification of apoferritin, ferritin, and other iron‐binding proteins.