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Role of β‐Xylosidase in Hemicellulose Hydrolysis by Xylanase from Penicillium funiculosum 1
Author(s) -
Seeta R.,
Deshpande Vasanti,
Rao Mala
Publication year - 1989
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1989.tb01402.x
Subject(s) - xylanase , hydrolysis , xylan , hemicellulose , chemistry , cellulase , ammonium sulfate precipitation , ammonium , ammonium sulfate , xylose , penicillium , food science , chromatography , biochemistry , enzyme , fermentation , organic chemistry , size exclusion chromatography
Penicillium funiculosum produces 16.7 and 0.2 Uml −1 of xylanase and xylosidase with traces of endoglucanase and filter paper activity when grown on a medium containing xylan. The rate of hydrolysis of xylan increased with the increasing levels of xylanase but was not strictly proportional to enzyme concentration. Fractions enriched in xylanase and xylosidase activity were obtained by fractional precipitation of culture broth with ammonium sulfate. Hydrolysis of xylan using varying amounts of xylosidase showed that the optimum ratio of xylanase to xylosidase for maximum hydrolysis is 7.14. The results on the decreased rate of hydrolysis, due to a further increase in xylosidase, are discussed.