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Improved stability of 2,3‐bisphosphoglycerate during storage of hexokinase‐overloaded erythrocytes
Author(s) -
Magnani M.,
Rossi L.,
Bianchi M.,
Serafini G.,
Zocchi E.,
Laguerre M.,
Ropars C.
Publication year - 1989
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1989.tb00069.x
Subject(s) - hexokinase , hemolysis , red blood cell , glycolysis , chemistry , enzyme , biochemistry , tonicity , microbiology and biotechnology , biology , immunology
Human red blood cells were overloaded with homogeneous human hexokinase using a procedure of encapsulation based on hypotonic hemolysis and isotonic resealing and reannealing to achieve a final activity that was 15 times higher than that in control cells. Storage for 5 weeks at 4 degrees C of hexokinase‐overloaded erythrocytes shows that these cells undergo small K+ leakage and mean cell volume increase compared with control cells. Furthermore, after these 5 weeks of storage the 2,3‐bisphosphoglycerate content was normal while the ATP concentration was slightly reduced. These results and other properties suggest that encapsulation of key glycolytic enzymes in erythrocytes can provide a new way to maintain in vitro functionally active red blood cells for at least 5 weeks.