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Applications of Monoclonal Antibodies to Human Follicle‐Stimulating Hormone in Enzyme Immunoassays
Author(s) -
Chen KW,
Chow SN,
Lee CY
Publication year - 1989
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1989.tb00051.x
Subject(s) - monoclonal antibody , immunoassay , horseradish peroxidase , microbiology and biotechnology , epitope , antibody , chemistry , hybridoma technology , glycoprotein , enzyme , biochemistry , biology , immunology
Monoclonal antibodies against human follicle‐stimulating hormone (hFSH) were generated by using an improved hybridoma technique with a semisolid medium in methylcellulose for initial cloning. The generated monoclonal antibodies were characterized with respect to their subunit and epitope specificity as well as cross‐reactivity to other glycoprotein hormones. Monoclonal antibodies of high affinity and high specificity to hFSH were finally selected for applications in sandwich enzyme immunoassay. The monoclonal antibody specific to the alpha‐subunit of FSH was coated on microtiter wells and served as the first antibody. The other high‐affinity monoclonal antibody specific to beta‐subunit of FSH was labeled with horseradish peroxidase and served as the second antibody. This immunoassay can be performed within 70 min at room temperature and has a minimum sensitivity of 2 mIU/ml for serum sample.