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Sensitive Assay of Cysteine Proteinases Using new Peptide P‐Nitroanilides
Author(s) -
Fukal L.,
Kasafirek E.
Publication year - 1988
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1988.tb00037.x
Subject(s) - papain , trypsin , chemistry , chymotrypsin , cysteine , peptide , hydrolysis , bromelain , biochemistry , enzyme
N‐Succinyl‐glycyl‐leucyl‐cystein(S‐benzyl) p‐nitroanilide and N‐succinyl‐leucyl‐leucyl‐cystein(S‐benzyl) p‐nitroanilide were found to be very sensitive substrates for the assay of papain, ficin, and bromelain. These p‐nitroanilides were hydrolyzed only very slightly by chymotrypsin, but not detectably by trypsin.