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Determination of Parameters for Enzyme Therapy Using L‐Asparaginase Entrapped in Canine Erythrocytes
Author(s) -
Naqi A.,
DeLoach JR,
Andrews K.,
Satterfield W.,
Keeling M.
Publication year - 1988
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1988.tb00026.x
Subject(s) - asparaginase , enzyme , in vivo , chemistry , in vitro , dialysis , efflux , inulin , cell , microbiology and biotechnology , chromatography , pharmacology , biochemistry , biology , medicine , immunology , lymphoblastic leukemia , leukemia
The antitumor agent L‐asparaginase was entrapped in canine erythrocytes by a single dialysis encapsulation (efficiency mean = 30%). Concentration of asparaginase in carrier cells was about 240 IU/ml, with an average of 62% cell recovery. Use of a double dialysis procedure increased the L‐asparaginase concentration within carrier cells to 530 IU/ml, with an overall cell recovery of 53.9%. In vitro efflux experiments showed L‐asparaginase‐loaded canine carriers were stable at both 4 and 37 degrees C for an 18‐h period. In vivo cell survival studies showed that carrier cells did circulate and that L‐asparaginase had a half‐life of 6.5 days. No evidence suggesting that the enzyme left the cell was found. Carrier cells prepared with [3H]inulin and [14C]sucrose were stored at 4 degrees C for 2 weeks and began to show signs of deterioration after 2 days.