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A Colorimetric Procedure to Monitor the Extent of Crosslinking of Proteins by Bisimidoesters
Author(s) -
Kamra A.,
Gupta MN
Publication year - 1988
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1988.tb00019.x
Subject(s) - ninhydrin , reagent , chemistry , sulfonic acid , colorimetry , chromatography , biochemistry , amino acid , organic chemistry , combinatorial chemistry
Reaction of proteins with bisimidoesters is one of the popular crosslinking techniques. The extent of the reaction cannot be followed directly using the commonly used free amino group‐determining reagents ninhydrin and 2,4,6‐trinitrobenzene sulfonic acid since they react with bisimidoesters. However, we found that the extent of amidination of protein could be followed colorimetrically by using 1‐fluoro‐2,4‐dinitrobenzene.