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Encapsulation of interleukin‐2 in murine erythrocytes and subsequent deposition in mice receiving a subcutaneous injection
Author(s) -
DeLoach JR,
Andrews K.,
Sheffield CL
Publication year - 1988
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1988.tb00013.x
Subject(s) - incubation , in vitro , subcutaneous injection , chemistry , recombinant dna , interleukin , microbiology and biotechnology , ratón , interleukin 2 , cytokine , immunology , biochemistry , biology , endocrinology , gene
Radiolabeled recombinant human interleukin‐2 (IL‐2) was successfully encapsulated in both mouse and sheep erythrocytes. Of the added IL‐2, 70% was recovered bound to or encapsulated within the carrier cells. Erythrocytes containing IL‐2 were stable in vitro and most of the IL‐2 remained associated with the cells following a 16‐h incubation at 37 degrees C. When carrier erythrocytes containing IL‐2 were injected subcutaneously into mice, intact [35S]IL‐2 was detectable in a number of tissues 3 days after injection.