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Thermally Modified Azocasein–A New Insoluble Substrate for the Determination of Proteolytic Activity
Author(s) -
Safarik I.
Publication year - 1987
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1987.tb00481.x
Subject(s) - chromogenic , substrate (aquarium) , bacillales , chemistry , chromatography , substrate specificity , bacillaceae , extracellular , proteolytic enzymes , biochemistry , bacteria , enzyme , biology , ecology , genetics , bacillus subtilis
An insoluble chromogenic substrate for the determination of proteolytic activity was prepared by heating azocasein in a thin layer at 200 degrees C for 4 h in a hot‐air thermostat. The activity of an extracellular bacterial proteinase produced by Bacillus sp. was determined with this new substrate.