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High‐performance liquid chromatographic separation and partial characterization of human protamines 1, 2, and 3
Author(s) -
Balhorn R.,
Corzett M.,
Mazrimas J.,
Stanker LH,
Wyrobek A.
Publication year - 1987
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1987.tb00465.x
Subject(s) - protamine , chromatography , amino acid , high performance liquid chromatography , electrophoresis , chemistry , protamine sulfate , peptide sequence , amino acid analysis , biochemistry , gene , heparin
A method for separating the three human protamines by HPLC of underivatized, total protamine extracts on a Nucleosil RP‐C18 column is described. The identities of the three proteins have been confirmed by a combination of disc gel electrophoresis, amino acid composition, and primary sequence analysis. The results show that human protamine 3 elutes first, closely followed by protamine 2. Protamine 1 elutes later. The amino acid compositions and partial amino terminal sequences of human protamines 2 and 3 indicate that these two proteins are very closely related and suggest that they differ only by three amino‐terminal amino acids.