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An attempt to determine lipid peroxides with polyethylene glycol‐modified hemin
Author(s) -
Ajima A.,
Cao SG,
Takahashi K.,
Matsushima A.,
Saito Y.,
Inada Y.
Publication year - 1987
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1987.tb00462.x
Subject(s) - chemistry , hemin , hydrogen peroxide , carbodiimide , organic chemistry , amide , peroxidase , microanalysis , polyethylene glycol , polymer chemistry , enzyme , heme
Hemin, having two carboxyl groups, was coupled with alpha‐(3‐aminopropyl)‐omega‐methoxypoly(oxyethylene) through the acid‐amide bond formed with carbodiimide. The modified hemin catalyzed the peroxidase reaction in 1,1,1‐trichloroethane using benzoyl peroxide or peroxides in unsaturated fatty acids as the hydrogen acceptor and leuco crystal violet as the hydrogen donor. A basic study on quantitative microanalysis of the lipid peroxides was attempted.