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The synthesis of peptides with potential thymic hormone activity: the synthesis of endo‐Arg38a‐deacetylthymosin beta 10
Author(s) -
Abiko T.,
Sekino H.
Publication year - 1987
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1987.tb00459.x
Subject(s) - trifluoroacetic acid , in vitro , peptide , cytotoxic t cell , beta (programming language) , thioanisole , biochemistry , amino acid , stereochemistry , chemistry , peptide sequence , biology , population , medicine , chromatography , gene , computer science , programming language , catalysis , environmental health
A tritetracontapeptide corresponding to the entire amino acid sequence of endo‐Arg38a‐deacetylthymosin beta 10 was synthesized by a conventional solution method. Seven peptide fragments were assembled, followed by deprotection with 1 M trifluoromethanesulfonic acid‐thioanisole‐Me2Se in trifluoroacetic acid. In preliminary experiments the synthetic tritetracontapeptide increased the entire peripheral T‐cell population and a helper T‐cell subset when incubated in vitro with blood which was obtained from a uremic patient with pneumonia, but a suppressor/cytotoxic T‐cell subset was unaffected under these conditions. The synthetic endo‐Arg38a‐deacetylthymosin beta 10 was as active as synthetic deacetylthymosin beta 10 in this in vitro assay.