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Initial characterization of shade avoidance response suggests functional diversity between Populus phytochrome B genes
Author(s) -
Karve Abhijit A.,
Jawdy Sara S.,
Gunter Lee E.,
Allen Sara M.,
Yang Xiaohan,
Tuskan Gerald A.,
Wullschleger Stan D.,
Weston David J.
Publication year - 2012
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2012.04288.x
Subject(s) - arabidopsis , biology , phenotype , phytochrome , gene , transgene , gene expression , microbiology and biotechnology , shade avoidance , genetics , epigenetics , mutant , transcription factor , botany , red light
Summary Shade avoidance signaling involves perception of incident red/far‐red (R/ FR ) light by phytochromes (PHYs) and modulation of downstream transcriptional networks. Although these responses are well studied in Arabidopsis , little is known about the role of PHYs and the transcriptional responses to shade in the woody perennial Populus . Tissue expression and subcellular localization of Populus PHYs was studied by quantitative real‐time PCR ( qRT ‐PCR) and protoplast transient assay. Transgenic lines with altered PHYB1 and/or PHYB2 expression were used in phenotypic assays and transcript profiling with qRT ‐PCR. RNA‐Seq was used to identify transcriptional responses to enriched FR light. All three PHY s were differentially expressed among tissue types and PHYB s were targeted to the nucleus under white light. Populus PHYB 1 rescued Arabidopsis phyB mutant phenotypes. Phenotypes of Populus transgenic lines and the expression of candidate shade response genes suggested that PHYB 1 and PHYB 2 have distinct yet overlapping functions. RNA ‐Seq analysis indicated that genes associated with cell wall modification and brassinosteroid signaling were induced under enriched FR light in Populus . This study is an initial attempt at deciphering the role of Populus PHYs by evaluating transcriptional reprogramming to enriched FR and demonstrates functional diversity and overlap of the Populus PHYB 1 and PHYB 2 in regulating shade responses.