Premium
StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst
Author(s) -
Kobayashi Michie,
Yoshioka Miki,
Asai Shuta,
Nomura Hironari,
Kuchimura Kazuo,
Mori Hitoshi,
Doke Noriyuki,
Yoshioka Hirofumi
Publication year - 2012
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2012.04226.x
Subject(s) - phytophthora infestans , biology , pseudomonas syringae , pathogen , blight , microbiology and biotechnology , reactive oxygen species , plant disease resistance , hypersensitive response , transgene , obligate , plant defense against herbivory , complementation , botany , gene , biochemistry , mutant
Summary• Potato ( Solanum tuberosum ) calcium‐dependent protein kinase (StCDPK5) has been shown to phosphorylate the N‐terminal region of plasma membrane RBOH (respiratory burst oxidase homolog) proteins, and participate in StRBOHB‐mediated reactive oxygen species (ROS) burst. The constitutively active form, StCDPK5VK, provides a useful tool for gain‐of‐function analysis of RBOH in defense responses. • StCDPK5‐ and StCDPK5VK‐green fluorescent protein fusion proteins were predominantly targeted to the plasma membrane, and conditional expression of StCDPK5VK activated StRBOHA‐D. The interaction was confirmed by bimolecular fluorescence complementation assay. We generated transgenic potato plants containing StCDPK5VK under the control of a pathogen‐inducible promoter to investigate the role of ROS burst on defense responses to blight pathogens. • Virulent isolates of the late blight pathogen Phytophthora infestans and the early blight pathogen Alternaria solani induced hypersensitive response‐like cell death accompanied by ROS production at the infection sites of transgenic plants. Transgenic plants showed resistance to the near‐obligate hemibiotrophic pathogen P. infestans and, by contrast, increased susceptibility to the necrotrophic pathogen A. solani . • These results indicate that RBOH‐dependent ROS contribute to basal defense against near‐obligate pathogens, but have a negative role in resistance or have a positive role in expansion of disease lesions caused by necrotrophic pathogens.