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A sensitive LC‐ESI‐Q‐TOF‐MS method reveals novel phytosiderophores and phytosiderophore–iron complexes in barley
Author(s) -
Tsednee Munkhtsetseg,
Mak YitWai,
Chen YetRan,
Yeh KuoChen
Publication year - 2012
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2012.04206.x
Subject(s) - siderophore , chemistry , electrospray ionization , hordeum vulgare , ferric , mass spectrometry , chromatography , metal , time of flight mass spectrometry , biochemistry , ionization , botany , inorganic chemistry , biology , organic chemistry , poaceae , ion , gene
Summary• The direct analysis of phytosiderophores (PSs) and their metal complexes in plants is critical to understanding the biological functions of different PSs. Here we report on a rapid and highly sensitive liquid chromatography‐electrospray ionization‐quadrupole‐time of flight‐mass spectrometry (LC‐ESI‐Q‐TOF‐MS) method for the direct and simultaneous determination of free PSs and their ferric complexes in plants. • In addition to previously reported PSs – deoxymugineic acid (DMA), mugineic acid (MA) and epihydroxymugineic acid (epi‐HMA) – two more PSs, avenic acid (AVA) and hydroxyavenic acid (HAVA), were identified by this method in roots of Hordeum vulgare cv Himalaya and in root exudates under iron (Fe) deficiency. • The two identified PSs could be responsible for Fe acquisition under Fe deficiency because of their relative abundance and ability to form ferric complexes in secreted root exudates. • This LC‐ESI‐Q‐TOF‐MS method greatly facilitates the identification of free PSs and PS–Fe complexes in one plant sample.