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Arabidopsis GT34 family contains five xyloglucan α‐1,6‐xylosyltransferases
Author(s) -
Vuttipongchaikij Supachai,
Brocklehurst David,
SteeleKing Clare,
Ashford David A.,
Gomez Leonardo D.,
McQueenMason Simon J.
Publication year - 2012
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2012.04196.x
Subject(s) - arabidopsis , gene , mutant , gene knockout , biology , genetics , arabidopsis thaliana , xyloglucan , gene family , genome , cell wall
Summary• The Arabidopsis genome includes seven family 34 glycosyltransferase (GT34) encoding genes. XXT1 and XXT2 have previously been shown to encode XyG α‐1,6‐xylosyltransferases, while knockout mutants of a third, XXT5 , exhibit decreased XyG content, suggesting a similar activity. Here, we extend the study to the rest of the Arabidopsis GT34 genes in terms of biochemical activity and their roles in XyG biosynthesis. • The enzyme activity of XXTs was investigated using recombinant protein expressed in E. coli . XyG analysis of single and double T‐DNA insertion knockouts, together with overexpression of GT34s in selected mutant lines, provided detailed function of each gene. • We reveal the activity of the third member of the GT34 gene family (XXT4) that exhibits xylosyltransferase activity. Double mutants for either xxt2 or xxt5 had a large impact on XyG content, structure and size distribution. Overexpression of the remaining member, XXT3 , was able to restore XyG epitopes in xxt2, xxt5 and xxt2 xxt5 double knockouts, suggesting that it also encodes a protein with XXT activity. • Our work demonstrates that five of the seven Arabidopsis GT34 genes encode XXT enzymes.