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Arabidopsis RAP2.2 plays an important role in plant resistance to Botrytis cinerea and ethylene responses
Author(s) -
Zhao Yang,
Wei Tong,
Yin KangQuan,
Chen Zhangliang,
Gu Hongya,
Qu LiJia,
Qin Genji
Publication year - 2012
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2012.04160.x
Subject(s) - mutant , botrytis cinerea , arabidopsis , ethylene , biology , microbiology and biotechnology , transgene , botrytis , transcription factor , gene , botany , genetics , biochemistry , catalysis
Summary• Ethylene plays a crucial role in plant resistance to necrotrophic pathogens, in which ETHYLENE RESPONSE FACTORs (ERFs) are often involved. • Here, we evaluated the role of an ERF transcription factor, RELATED TO AP2 2 (RAP2.2), in Botrytis resistance and ethylene responses in Arabidopsis . We analyzed the resistance of transgenic plants overexpressing RAP2.2 and the T‐DNA insertion mutant to Botrytis cinerea . We assessed its role in the ethylene signaling pathway by molecular and genetic approaches. • RAP2.2 ‐overexpressing transgenic plants showed increased resistance to B. cinerea , whereas its T‐DNA insertion mutant rap2.2‐3 showed decreased resistance. Overexpression of RAP2.2 in ethylene insensitive 2 ( ein2 ) and ein3 ein3‐like 1 ( eil1 ) mutants restored their resistance to B. cinerea . Both ethylene and Botrytis infection induced the expression of RAP2.2 and the induction was disrupted in ein2 and ein3 eil1 mutants. We identified rap2.12‐1 as a T‐DNA insertion mutant of RAP2.12 , the closest homolog of RAP2.2 . The hypocotyls of rap2.2‐3 rap2.12‐1 double mutants showed ethylene insensitivity. The constitutive triple response in constitutive triple response1 ( ctr1 ) was partially released in the rap2.2‐3 rap2.12‐1 ctr1 triple mutants. • Our findings demonstrate that RAP2.2 functions as an important regulator in Botrytis resistance and ethylene responses.