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Spatially resolved analysis of small molecules by matrix‐assisted laser desorption/ionization mass spectrometric imaging (MALDI‐MSI)
Author(s) -
Peukert Manuela,
Matros Andrea,
Lattanzio Giuseppe,
Kaspar Stephanie,
Abadía Javier,
Mock HansPeter
Publication year - 2012
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2011.03970.x
Subject(s) - mass spectrometry imaging , maldi imaging , mass spectrometry , chemistry , matrix assisted laser desorption/ionization , hordeum vulgare , small molecule , nicotiana tabacum , matrix (chemical analysis) , reproducibility , sample preparation , desorption , analytical chemistry (journal) , biological system , chromatography , botany , biology , biochemistry , organic chemistry , poaceae , adsorption , gene
Summary• Matrix‐assisted laser desorption/ionization mass spectrometric imaging (MALDI‐MSI) of tissues provides the means to analyse the spatial distributions of small molecules and proteins within tissues. This imaging technique is commonplace in medicinal and pharmaceutical research, but its application in plant science is very recent. Broader introduction requires specific adaptations for plant tissues. Sample preparation is of paramount importance in order to obtain high‐quality spectra providing sufficient spatial resolution for compounds. Optimization is required for sectioning, choice of matrix and means of matrix deposition. • Here, we present our current protocols for the detection of small molecules in cryodissected immature barley ( Hordeum vulgare ) grains and tobacco ( Nicotiana tabacum ) roots. • Examples of MALDI‐MSI measurements are provided, and the level of reproducibility across biological replicates is addressed. Furthermore, our approaches for the validation of distribution patterns and for the identification of molecules are described. • Finally, we discuss how MALDI‐MSI can contribute to applied plant research.