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OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice ( Oryza sativa )
Author(s) -
Qi YanHua,
Wang SuiKang,
Shen ChenJia,
Zhang SaiNa,
Chen Yue,
Xu YanXia,
Liu Yu,
Wu YunRong,
Jiang DeAn
Publication year - 2012
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2011.03910.x
Subject(s) - auxin , oryza sativa , elongation , transcription factor , biology , microbiology and biotechnology , activator (genetics) , gene expression , genetically modified rice , regulation of gene expression , gene , botany , biochemistry , genetically modified crops , transgene , materials science , ultimate tensile strength , metallurgy
Summary• Auxin has an important role in maintaining optimal root system architecture (RSA) that can cope with growth reductions of crops caused by water or nutrient shortages. However, the mechanism of controlling RSA remains largely unclear. Here, we found a limiting factor of RSA – OsARF12 – an auxin response factor whose knockout led to decreased primary root length in rice ( Oryza sativa ). • OsARF12 as a transcription activator can facilitate the expression of the auxin response element DR5::GFP, and OsARF12 was inhibited by osa‐miRNA167d by transient expression in tobacco and rice callus. • The root elongation zones of osarf12 and osarf12/25 , which had lower auxin concentrations, were distinctly shorter than for the wild‐type, possibly as a result of decreased expression of auxin synthesis genes OsYUCCA s and auxin efflux carriers OsPIN s and OsPGP s. The knockout of OsARF12 also altered the abundance of mitochondrial iron‐regulated ( OsMIR ), iron (Fe)‐regulated transporter1 ( OsIRT1 ) and short postembryonic root1 ( OsSPR1 ) in roots of rice, and resulted in lower Fe content. • The data provide evidence for the biological function of OsARF12, which is implicated in regulating root elongation. Our investigation contributes a novel insight for uncovering regulation of RSA and the relationship between auxin response and Fe acquisition.