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The Arabidopsis Ca 2+ ‐dependent protein kinase CPK12 negatively regulates abscisic acid signaling in seed germination and post‐germination growth
Author(s) -
Zhao Rui,
Sun HaiLi,
Mei Chao,
Wang XiaoJing,
Yan Lu,
Liu Rui,
Zhang XiaoFeng,
Wang XiaoFang,
Zhang DaPeng
Publication year - 2011
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2011.03793.x
Subject(s) - arabidopsis , abscisic acid , arabidopsis thaliana , signal transduction , biology , microbiology and biotechnology , germination , kinase , transcription factor , protein kinase a , biochemistry , botany , gene , mutant
Summary• Ca 2+ ‐dependent protein kinase (CDPK) is believed to be involved in abscisic acid (ABA) signaling, and several members of the Arabidopsis CDPK superfamily have been identified as positive ABA signaling regulators, but it remains unknown if CDPK negatively regulates ABA signaling. • Here, we investigated the function of an Arabidopsis ( Arabidopsis thaliana ) CDPK, CPK12, in ABA signaling pathway. • We generated Arabidopsis CPK12 ‐RNAi lines, and observed that downregulation of CPK12 resulted in ABA hypersensitivity in seed germination and post‐germination growth, and altered expression of a set of ABA‐responsive genes. Expression assay showed that CPK12 was ubiquitously expressed and localized to both cytosol and nucleus. Biochemical assays showed that CPK12 interacted with, phosphorylated and stimulated a type 2C protein phosphatase ABI2, and phosphorylated two ABA‐responsive transcription factors (ABF1 and ABF4) in vitro . • Our findings show that the Arabidopsis CPK12 is a negative ABA‐signaling regulator in seed germination and post‐germination growth, suggesting that different members of the CDPK family may constitute a regulation loop by functioning positively and negatively in ABA signal transduction.