z-logo
Premium
The moss Physcomitrella patens contains cyclopentenones but no jasmonates: mutations in allene oxide cyclase lead to reduced fertility and altered sporophyte morphology
Author(s) -
Stumpe Michael,
Göbel Cornelia,
Faltin Bernd,
Beike Anna K.,
Hause Bettina,
Himmelsbach Kiyoshi,
Bode Julia,
Kramell Robert,
Wasternack Claus,
Frank Wolfgang,
Reski Ralf,
Feussner Ivo
Publication year - 2010
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2010.03406.x
Subject(s) - physcomitrella patens , allene , jasmonic acid , mutant , chemistry , jasmonate , biology , biochemistry , botany , gene , arabidopsis , catalysis
Summary• Two cDNAs encoding allene oxide cyclases (PpAOC1, PpAOC2), key enzymes in the formation of jasmonic acid (JA) and its precursor (9 S ,13 S )‐12‐oxo‐phytodienoic acid ( cis ‐(+)‐OPDA), were isolated from the moss Physcomitrella patens . • Recombinant PpAOC1 and PpAOC2 show substrate specificity against the allene oxide derived from 13‐hydroperoxy linolenic acid (13‐HPOTE); PpAOC2 also shows substrate specificity against the allene oxide derived from 12‐hydroperoxy arachidonic acid (12‐HPETE). • In protonema and gametophores the occurrence of cis ‐(+)‐OPDA, but neither JA nor the isoleucine conjugate of JA nor that of cis ‐(+)‐OPDA was detected. • Targeted knockout mutants for PpAOC1 and for PpAOC2 were generated, while double mutants could not be obtained. The ΔPpAOC1 and ΔPpAOC2 mutants showed reduced fertility, aberrant sporophyte morphology and interrupted sporogenesis.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here