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Suppression of the AvrBs1‐specific hypersensitive response by the YopJ effector homolog AvrBsT from Xanthomonas depends on a SNF1‐related kinase
Author(s) -
Szczesny Robert,
Büttner Daniela,
Escolar Lucia,
Schulze Sebastian,
Seiferth Anja,
Bonas Ulla
Publication year - 2010
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2010.03346.x
Subject(s) - effector , xanthomonas , hypersensitive response , kinase , biology , microbiology and biotechnology , bacteria , gene , biochemistry , genetics , plant disease resistance
Summary• Pathogenicity of the Gram‐negative plant pathogen Xanthomonas campestris pv. vesicatoria ( Xcv ) depends on a type III secretion system that translocates a cocktail of > 25 type III effector proteins into the plant cell. • In this study, we identified the effector AvrBsT as a suppressor of specific plant defense. AvrBsT belongs to the YopJ/AvrRxv protein family, members of which are predicted to act as proteases and/or acetyltransferases. • AvrBsT suppresses the hypersensitive response (HR) that is elicited by the effector protein AvrBs1 from Xcv in resistant pepper plants. HR suppression occurs inside the plant cell and depends on a conserved predicted catalytic residue of AvrBsT. Yeast two‐hybrid based analyses identified plant interaction partners of AvrBs1 and AvrBsT, including a putative regulator of sugar metabolism, SNF1‐related kinase 1 (SnRK1), as interactor of AvrBsT. Intriguingly, gene silencing experiments revealed that SnRK1 is required for the induction of the AvrBs1‐specific HR. • We therefore speculate that SnRK1 is involved in the AvrBsT‐mediated suppression of the AvrBs1‐specific HR.