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MicroRNA expression profile in common bean ( Phaseolus vulgaris ) under nutrient deficiency stresses and manganese toxicity
Author(s) -
ValdésLópez Oswaldo,
Yang S. Samuel,
AparicioFabre Rosaura,
Graham Peter H.,
Reyes José Luis,
Vance Carroll P.,
Hernández Georgina
Publication year - 2010
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2010.03320.x
Subject(s) - phaseolus , microrna , biology , gene expression , gene , genetics , botany
Summary• MicroRNAs (miRNAs) play a pivotal role in post‐transcriptional regulation of gene expression in plants. Information on miRNAs in legumes is as yet scarce. This work investigates miRNAs in an agronomically important legume, common bean ( Phaseolus vulgaris ).• A hybridization approach employing miRNA macroarrays – printed with oligonucleotides complementary to 68 known miRNAs – was used to detect miRNAs in the leaves, roots and nodules of control and nutrient‐stressed (phosphorus, nitrogen, or iron deficiency; acidic pH; and manganese toxicity) common bean plants. • Thirty‐three miRNAs were expressed in control plants and another five were only expressed under stress conditions. The miRNA expression ratios (stress:control) were evaluated using principal component and hierarchical cluster analyses. A group of miRNAs responded to nearly all stresses in the three organs analyzed. Other miRNAs showed organ‐specific responses. Most of the nodule‐responsive miRNAs showed up‐regulation. miRNA blot expression analysis confirmed the macroarray results. Novel miRNA target genes were proposed for common bean and the expression of selected targets was evaluated by quantitative reverse transcriptase–polymerase chain reaction. • In addition to the detection of previously reported stress‐responsive miRNAs, we discovered novel common bean stress‐responsive miRNAs, for manganese toxicity. Our data provide a foundation for evaluating the individual roles of miRNAs in common bean.