Premium
Differential sensitivity of the Arabidopsis thaliana transcriptome and enhancers to the effects of genome doubling
Author(s) -
Pignatta Daniela,
Dilkes Brian P.,
Yoo SukYoung,
Henry Isabelle M.,
Madlung Andreas,
Doerge Rebecca W.,
Jeffrey Chen Z.,
Comai Luca
Publication year - 2010
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2010.03198.x
Subject(s) - biology , enhancer , arabidopsis , arabidopsis thaliana , gene , genetics , transcriptome , genome , transgene , microarray analysis techniques , ploidy , gene expression , gene duplication , gene dosage , mutant
Summary• Two fundamental types of polyploids are known: allopolyploids, in which different parental chromosome sets were combined by ancestral hybridization and duplication; and autopolyploids, which derive from multiplication of the same chromosome set. In autopolyploids, changes to the nuclear environment are not as profound as in allopolyploids, and therefore the effects of genome doubling on gene regulation remain unclear. • To investigate the consequences of autopolyploidization per se , we performed a microarray analysis in three equivalent lineages of matched diploids and autotetraploids of Arabidopsis thaliana. Additionally, we compared the expression levels of GFP transgenes driven by endogenous enhancer elements (enhancer traps) in diploids and autotetraploid of 16 transgenic lines. • We expected that true ploidy‐dependent changes should occur in independently derived autopolyploid lineages. By this criterion, our microarray analysis detected few changes associated with polyploidization, while the enhancer‐trap analysis revealed altered GFP expression at multiple plant life stages for 25% of the lines tested. Genes on individual traps were coordinately regulated while endogenous gene expression was not affected except for one line. • The unique sensitivity of enhancer traps to ploidy, in contrast to the observed stability of genes, could derive from lower complexity of regulatory pathways acting on traps versus endogenous genes.