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Mobilization of retrotransposons in synthetic allotetraploid tobacco
Author(s) -
Petit M.,
Guidat C.,
Daniel J.,
Denis E.,
Montoriol E.,
Bui Q. T.,
Lim K. Y.,
Kovarik A.,
Leitch A. R.,
Grandbastien MA.,
Mhiri C.
Publication year - 2010
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.2009.03140.x
Subject(s) - retrotransposon , transposable element , biology , nicotiana tabacum , genetics , genome , indel , long terminal repeat , gene , genotype , single nucleotide polymorphism
Summary• Allopolyploidy is a major driving force in plant evolution and can induce rapid structural changes in the hybrid genome. As major components of plant genomes, transposable elements are involved in these changes. In a previous work, we observed turnover of retrotransposon insertions in natural allotretraploid tobacco ( Nicotiana tabacum ). Here, we studied the early stages of allopolyploid formation by monitoring changes at retrotransposon insertion sites in the Th37 synthetic tobacco. • We used sequence‐specific amplification polymorphism (SSAP) to study insertion patterns of two populations of the Tnt1 retrotransposon in Th37 S4 generation plants, and characterized the nature of polymorphic insertion sites. • We observed significant amplification of young Tnt1 populations. Newly transposed copies were amplified from maternal elements and were highly similar to Tnt1A tobacco copies amplified in response to microbial factors. A high proportion of paternal SSAP bands were not transmitted to the hybrid, corresponding to various rearrangements at paternal insertion sites, including indels or the complete loss of the Tnt1/flanking junction. • These data indicate that major changes, such as retrotransposon amplification and molecular restructuring in or around insertion sites, occur rapidly in response to allopolyploidy.